Mitochondrial Hexokinase

Mitochondrial hexokinase from small-intestinal mucosa and brain.

1. The submitochondrial localization of hexokinase activity in preparations of mitochondria from the small intestine of the guinea pig was studied by conventional methods. 2. Hexokinase activity in this tissue was predominantly associated with the outer mitochondrial membrane. 3. The inactivation of mitochondrial enzymes by trypsin in iso-osmotic and hypo-osmotic conditions was also used to det...

Subcellular localization of hexose kinases in pea stems: mitochondrial hexokinase.

The subcellular localization of hexose phosphorylating activity in extracts of pea stems has been studied by differential centrifugation and sucrose density gradient centrifugation. The hexokinase (EC 2.7.1.1) was associated with the mitochondria, whereas fructokinase (EC 2.7.1.4) was in the cytosolic fraction. Some properties of the mitochondrial hexokinase were studied. The enzyme had a high ...

Complexes between hexokinase, mitochondrial porin and adenylate translocator in brain: regulation of hexokinase, oxidative phosphorylation and permeability transition pore.

Introduction Hexokinase I binds to the mitochondrial surface of brain [l], muscle [2], fat [3], kidney [4] and liver cells [S]. Responsibility for this association lies with a specific binding protein [6], which has been identified as mitochondrial porin in the outer membrane [7,8] and is also known as voltage-dependent anion channel W A C ) [9]. The isolated outer membrane pore, when reconstit...

A COMPARATIW STUDY ON THE BINDING OF HEXOKINASE TO THE OUTER MITOCHONDRIAL MEMBRANE AND A HYDROPHOBIC MATRIX

Effect of different thyroid states on mitochondrial porin synthesis and hexokinase activity in developing rabbit brain.

Voltage-dependent anion-selective channel proteins (VDACs) are pore-forming proteins found in the outer mitochondrial membrane of all eukaryotes and in brain postsynaptic membranes. VDACs regulate anion fluxes of a series of metabolites including ATP, thus regulating mitochondrial metabolic functions. Hexokinase binds to porin. The mitochondrially bound hexokinase can greatly increase the rate ...